A PolScope evaluation of meiotic spindle dynamics in frozen–thawed oocytes
نویسندگان
چکیده
منابع مشابه
P-103: Polscope Analysis of Meiotic Spindle and Zona Pellucida Birefringence of Metaphase II Oocytes in Polycystic Ovarian Syndrome Patients
Background Polycystic ovarian syndrome (PCOs) is one of the most common causes of infertility in women of reproductive age. Currently, one of the best therapeutic options for PCOs patients is ICSI. Moreover, IVM can be a useful technique for women with PCOs who are at the risk of ovarian hyperstimulation syndrome (OHSS). On the other hand, the oocyte quality can be a determining factor for outc...
متن کاملPolscope analysis of meiotic spindle changes in living metaphase II human oocytes during the freezing and thawing procedures.
BACKGROUND The clinical efficacy of the current methods used for cryopreservation of metaphase II human oocytes is low. Meiotic spindle disorders are thought to be largely responsible for this situation. METHODS Supernumerary fresh metaphase II human oocytes were cryopreserved in 1,2-propanediol with 0.1 M sucrose using a slow freezing/rapid thawing programme. Meiotic spindles were analysed i...
متن کاملThe Influence of Meiotic Spindle Configuration by Cysteamine during in vitro Maturation of Mouse Oocytes
Background: The aim of this study was to assess effects of cysteamine as an anti-oxidant on rate of in vitro maturation of oocyte and determination of its effects on spindle size and shape. Methods: Pre-mature mice were primed with pregnant mare stimulating gonadotrophin (PMSG) and germinal vesicle (GV) stage oocytes were obtained 48 h after. The oocytes were cultured in tissue culture medium (...
متن کاملMeiotic spindle assembly and chromosome segregation in oocytes
611 The Rockefeller University Press $30.00 J. Cell Biol. Vol. 215 No. 5 611–619 https://doi.org/10.1083/jcb.201607062 Introduction Sexual reproduction relies on the fusion of paternal and maternal haploid gametes—the sperm and the extremely large oocyte, respectively—forming a new diploid organism. Meiotic divisions contribute solely to the formation of haploid gametes. They consist of two suc...
متن کاملMeiotic spindle assembly and chromosome segregation in oocytes
Oocytes accumulate maternal stores (proteins, mRNAs, metabolites, etc.) during their growth in the ovary to support development after fertilization. To preserve this cytoplasmic maternal inheritance, they accomplish the difficult task of partitioning their cytoplasm unequally while dividing their chromosomes equally. Added to this complexity, most oocytes, for reasons still speculative, lack th...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2009
ISSN: 1472-6483
DOI: 10.1016/s1472-6483(10)60071-5